If you want to get the most out of your peptides, then you must learn how to handle and store them properly. Most of the time, you will receive your peptides from the manufacturers or vendors in lyophilized form, and they will always be accompanied with guidelines on how to store and handle them correctly. Here is a brief guide on what you need to do:
How to store lyophilized peptides
The stability of every peptide will be unique and dependent on its sequence. For lyophilized peptides, the recommended storage is at temperatures of -20 degrees Celsius, and away from direct sunlight. Under these conditions, lyophilized peptides can maintain their integrity for very many years. However, there are certain residues of amino acids on the peptide sequence, which may hinder the long-term stability.
How to store peptides in solution form
Peptides in solution form have a very short shelf-life compared to peptides in lyophilized forms. Additionally, such peptides are susceptible to bacterial degradation, and as such, it is recommended that peptides should never be stored in this form.
However, if storage in solution form cannot be avoided, then sterile buffers at 5-6pH should be dissolved in the peptides, and then the solution be divided into aliquots, which are then stored at a temperature of -20 degrees Celsius. Freeze-thaw cycles should be avoided with the aliquots.
How to prepare peptides for use
When getting your peptides ready for use in an assay or experiment, use the guidelines below:
- Remove the peptide vial from storage and warm to room temperature
- Quickly weigh the precise amount you want to use
- Reseal the vial immediately to avoid unnecessary contamination
- Return the remaining peptide back to storage, usually below -20 degrees Celsius and under dry conditions, away from direct sunlight or strong light.